26 patients died within 28 days (10.11%). Methods: FCER1A and HLA-DRA expression levels were quantified by droplet digital PCR in blood of 257 infected surgical patients. Here we studied the association between FCER1A expression and mortality in infected surgical patients. In a previous study, we evidenced that FCER1A (Fc Fragment Of IgE Receptor Ia) is the gene showing the lowest expression levels of the entire transcriptome in sepsis. The decreased expression of human leucocyte antigen (HLA)-DRA is proposed as a major feature of immunodepression and its persistent decrease is associated with mortality in sepsis. It is necessary to develop biomarkers of immune dysfunction that could help to identify patients at risk of poor outcomes. Introduction: Increasing evidence supports a central role for “immunosuppression” in sepsis.
P002 Depressed expression of FCER1A gene is associated with increased mortality in infected surgical patients R Almansa 1, C Andrés 2, M Martín-Fernández 3, S Montero 4, C Jambrina 5, C Doncel 6, J Sánchez-Crespo 5, M Heredia-Rodríguez 7, J Rico 4, C González 8, E Sánchez-Barrado 5, M Lorenzo-López 7, S Martín 4, L Muñoz-Bellvis 8, M Vaquero 5, E Tamayo 7, C Aldecoa 4, J Bermejo-Martín 6 1Hospital Clínico Universitario de Valladolid/IECSCYL, BioSepsis (Group of Biomedical Research in Sepsis), Valladolid, Spain 2Hospital Clínico Universitario de Valladolid, Clinical Analysis Service, Valladolid, Spain 3Hospital Clínico Universitario de Valladolid/IECSCYL, BioSepsis (Group for Biomedical Research in Sepsis), Valladolid, Spain 4Hospital Universitario Rio Hortega, Anesthesiology and Reanimation Service, Valladolid, Spain 5Hospital Clínico Universitario de Salamanca, Anesthesiology and Reanimation Service, Salamanca, Spain 6Hospital Clínico Universitario de Valladolid/IECSCYL, BioSepsis (Group for Biomedical Research in Sepsis), Valladolid, Spain 7Hospital Clínico Universitario de Valladolid, Anesthesiology and Reanimation Service, Valladolid, Spain 8Hospital Clínico Universitario de Salamanca, Department of General and Gastrointestinal Surgery, Salamanca, Spain Although there was a trend to preferential survival of sepsis patients with genotype C AQP5 despite the source of infection, only patients with AQP5 CC or CA genotype and abdominal sepsis (Sepsis-3), or a subgroup of the same AQP5 genotype experiencing septic shock, demonstrated increased 30-day survival versus AA homozygotic patients (P<0.002).Ĭonclusions: The informative value of detecting the AQP5 CC or CA genotype for prognosis of 30-day survival versus AA homozygotic patients is increased only in abdominal sepsis patients. Results: Distribution of alleles (A and C) and genotypes (AA, CA and CC) AQP5 1364A/C in patients with sepsis or sepsis subgroups (sepsis with no septic shock and sepsis shock patients) versus control group (healthy volunteers) did not differ. Data were analyzed by Kaplan-Meyer plot and Fisher test, and odds ratios were calculated. AQP5 polymorphism was studied by analyzing PCR products in a 2% agarose gel using a AQP5 1364A/C specific tetra primer set. Study groups (n=152) included ICU patients with abdominal sepsis (AS, including pancreatitits, peritonitis, cholecystitis, appendicitis n=98) and sepsis patients with other sources of infections. Methods: Sepsis and septic shock were defined in patients according to SEPSIS-3 (2016) recommendations. To increase the informative value of the prediction and decrease the cost, it might be crucial to determine at a pre-test level the subset of patients who might benefit most from the prognostic genotyping. Studies by Adamzik and colleagues have demonstrated that aquaporin AQP5 polymorphism (1364A/C, rs3759129) associates with increased 30-day survival in sepsis patients presumably due to increased gene expression that enhance the leukocyte migration. Introduction: The purpose of the study was to determine whether the preferential localization of the infection and age affect the prognostic value of the genetic marker AQP5 (1364A/C, rs3759129) in outcome prediction in sepsis patients. P001 Prognostic value of a genetic polymorphism of AQP5 in sepsis depends on a source of infection V Pisarev 1, A Chumachenko 1, I Tyurin 2, R Cherpakov 2, A Tutelyan 3 1Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A.Negovsky Institute of General Reanimatology, Moscow, Russia 2V.M.Buiyanov City Clinical Hospital, Anesthesia-Reanimatology Department, Moscow, Russia 3Central Institute of Epidemiology, Moscow, Russia
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